Tottenham manager Tim Sherwood accused Benfica coach Jorge Jesus of "lacking class" following Spurs'3-1 Europa League defeat.
Portuguese Jesus celebrated his side's third goal in front of the Spurs boss by raising three fingers in apparent reference to the score.
Sherwood confronted Jesus and the pair did not shake hands at full-time.
Sherwood said: "I thought his team were very good, got a lot of class. It's a pity he doesn't."
The result leaves Spurs struggling to qualify for the quarter-finals, and caps a difficult few days in which the manager said many of his players were playing for their futures, following the 4-0 thrashing by Chelsea in the Premier League on Saturday.
"In the first two minutes he was coming over to the fourth official saying I was stepping in his box," said Sherwood, who argued with Jesus throughout the match.
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"He doesn't mind himself, does he? Waving goodbye like that with three fingers? It lacks class. Why would anyone do that?
"He's got a good side, but [his behavious is] not for me, thank you. I have no intention of speaking to him."
Jesus said the three-fingered gesture referred to goalscorer Luisao, although the Brazilian wears number four.
"I was saying, 'number three, Luisao, number three'," he said. "Maybe here in England the benches are too close together, compared to in Portugal.
"In Portugal, we have more space apart and we can demonstrate ourselves in our own way.
"When at some stage I invaded Tim Sherwood's personal space, he told me to go to mine. He did the same to me. But it was no more than that; it was quite pleasant in the end."
Sherwood, who replaced Andre Villas-Boas in December, was pleased with his players' response after their defeat at Chelsea, ahead of the visit of Arsenal in the league on Sunday.
"[There was] no problem with the desire and effort tonight," he said. "That's what I questioned and asked for. We've got to see it over a consistent basis so I've got no complaints about that.
"It's a blow. I've got no problem with the attitude or desire; it's just we have to hold our hands up and say they're a class above.
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